Read e-book online A Litigator's Guide to DNA PDF

By Ron C. Michaelis

ISBN-10: 0123740363

ISBN-13: 9780123740366

A Litigator's consultant to DNA educates litigators, judges, criminalists, scholars, and others approximately all points of using DNA facts in legal and civil trials. It contains discussions of the molecular organic foundation for the checks, crucial laboratory practices, likelihood thought and mathematical calculations. It offers matters suitable to all events concerned with attempting a case, from the prosecution and the protection, and to the pass judgement on and jury. The ebook can also be super important as a textual content for college kids intending to careers in forensic technology and felony law.
The authors offer an entire heritage on either the molecular biology and the mathematical conception in the back of forensic exams written in particular for individuals with very little technological know-how history. No different e-book relates the foundational details on molecular biology and facts to criminal perform matters as commonly as this publication does.
* "Recapping the most aspect" bins supply an easy and concise precis of the most points
* contains a thesaurus of crucial phrases and their definitions
* encompasses a full-color insert with illustrations that emphasize key recommendations

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Additional info for A Litigator's Guide to DNA

Sample text

In any large population there are only 2–3 alleles at each of these loci, so the discriminative power of each individual test is limited. Combining the tests into one convenient assay, however, created a test that was faster and less labor-intensive than the VNTR tests, but provided a reasonable degree of discriminability. In addition, as discussed in Chapter 2, the PCR-based tests such as DQA1 and the PolymarkerTM test can be used to analyze some samples that cannot be analyzed by VNTR methods because they contain minute amounts of DNA or DNA that has been degraded.

The process is referred to as amplification of the target sequence, and the amplified DNA fragment is referred to as the amplicon. In order to understand what PCR does for the forensic investigator, consider how small a portion of the human DNA molecule the analyst examines at any given time. The human genome consists of 3 billion basepairs (3,000,000,000 bp) of DNA. If you want to analyze an STR, you are trying to look at a 200–400 bp stretch of sequence. A 300-bp target represents a mere one ten-millionth of the human genome.

Both the membrane-immobilized DNA and the probe DNA are denatured, so they are both single-stranded and looking for complementary sequences to which they can bind. The assay uses a chemical detection method to visualize how much of the probe has bound to the sample DNA. The analyst sees a band on the membrane wherever the probe DNA has bound. 1). Several control DNA samples, each having a different known concentration, are immobilized on the membrane as well, and each sample is quantified by comparing the intensity obtained from the sample with the intensities of the signals obtained from the control samples.

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A Litigator's Guide to DNA by Ron C. Michaelis


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